Detection of equine arteritis virus (EAV) by polymerase chain reaction (PCR) and differentiation of EAV strains by restriction enzyme analysis of PCR products
نویسندگان
چکیده
منابع مشابه
Increasing detection of polymerase chain reaction (PCR) by isolation of PCR products (IPCRp).
AIM To develop a method for enhanced polymerase chain reaction (PCR) product detection. METHODS During the PCR, the double-stranded product is generated with fluorescent dye on one strand, and biotin on the other strand. The product is captured on the streptavidin-coated plates with high efficiency (IPCRp). Washing of the all unamplified compounds, including dye-labeled unincorporated primers...
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ژورنال
عنوان ژورنال: Archives of Virology
سال: 1995
ISSN: 0304-8608,1432-8798
DOI: 10.1007/bf01322675